RS_Subreads Protocol

Use this protocol to filter reads based on the minimum read length and read quality you specified.

• No additional analysis is performed.
• Note: This protocol replaces the RS_Filter_Only protocol in previous releases.

Filtering Parameters (SFilter v1)

• Minimum Subread Length: Subreads shorter than this value (in base pairs) are filtered out and excluded from analysis.
• Minimum Polymerase Read Quality: Polymerase reads with lower quality than this value are filtered out and excluded from analysis.
• Minimum Polymerase Read Length: Polymerase reads shorter than this value (in base pairs) are filtered out and excluded from analysis.

Barcode Parameters (Barcode Module v1)

• My Library has DNA Barcodes That Are:
• Paired: A pair of barcode sequences that always occur together, and are different on each end.
• Symmetric: A single barcode sequence that occurs at both ends of the insert sequence.
• Barcode FASTA FIle: Path to the FASTA file containing the barcodes to use.
• Minimum Barcode Score: The minimum score for calling a barcode. This must be between 0 and 2 times the length of the barcode.